This project aims to use cutting-edge electron microscopy techniques to get a clearer look at how prions interact with their surroundings inside cells and on extracellular vesicles (EVs) which are released from cells. These interactions could play a major role in how prion diseases cause damage. While previous studies showed that prions can distort cell membranes, they lacked the fine detail needed to see exactly how this happens. Using advanced tools like cryo-electron microscopy and 3D imaging, this study will visualize prions in their natural environment in infected mouse neurons and derived EVs at a much higher resolution. This work is important because understanding how prions affect cells and spread via EVs can provide new insights into prion disease mechanism.