Dr. Graham S. Jackson
Grant Title:
“Development of a quantitative, real-time Protein-Misfolding by Cyclic Amplification (PMCA) reaction”
Location:
Medical Research Council (MRC) Prion Unit at University College London
Grant Year:
2019
Recent advances in the methods that support prion replication in vitro have allowed the introduction of genetically engineered prion proteins into active prion particles. By introducing modified fluorescent substrates into protein misfolding by cyclic amplification (PMCA), investigators hope to monitor prion replication, in real time, using fluorescence anisotropy. This will allow investigators to study the early stages of prion replication and provide quantitative kinetic measurements of fibril elongation. The improved resolution of such a real-time PMCA reaction will offer improved detection of prions for diagnosis and the potential to differentiate between strains by their replication kinetics.
Dr. Jackson is the recipient of:
- The Joanne (Jody) Atchison Memorial Grant, contributed by Dick Atchison and Family
- The Cheryl Molloy Memorial Grant, contributed by Tim Molloy and Family
- A Bequest from Beverly Nylund Huchala, in Memory of Peter Huchala
- The Tom Stivison Memorial Grant, contributed by Sandra (Cookie) Stivison
- The Strides for CJD Grant, contributed by the Families of the CJD Foundation