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Dr. Graham S. Jackson

Grant Title: Development of a quantitative, real-time Protein-Misfolding by Cyclic Amplification (PMCA) reaction Location: Medical Research Council (MRC) Prion Unit at University College London Grant Year: 2019


Recent advances in the methods that support prion replication in vitro have allowed the introduction of genetically engineered prion proteins into active prion particles. By introducing modified fluorescent substrates into protein misfolding by cyclic amplification (PMCA), investigators hope to monitor prion replication, in real time, using fluorescence anisotropy. This will allow investigators to study the early stages of prion replication and provide quantitative kinetic measurements of fibril elongation. The improved resolution of such a real-time PMCA reaction will offer improved detection of prions for diagnosis and the potential to differentiate between strains by their replication kinetics.

Recipient of:

The Strides for CJD Research Grant

Contributed by: The Families of the CJD Foundation

Funds raised by the annual Strides for CJD run/walk have been applied to research grants awarded since 2016.